Resources
Oligonucleotide properties calculator
Length, base composition, molecular weight, extinction coefficient, OD conversions, three melting-temperature methods and self-complementarity checks. Everything is calculated in your browser — the sequence is never uploaded.
Sequence
0 bases. Degenerate IUPAC codes are accepted.
Properties
Length
0 nt
GC content
0.0 %
Composition
A0 C0 G0 T0
MW single strand
0.0 g/mol
MW double strand
0.0 g/mol
Extinction coeff.
0 M⁻¹cm⁻¹
µg per OD₂₆₀
0.0
nmol per OD₂₆₀
0.00
Hairpin score
0
Self-dimer score
0
Hairpin and self-dimer scores are complementarity counts — higher means more structure. Anything above about 8 is worth redesigning.
Melting temperature
Basic (Wallace / GC)
0.0 °C
Salt adjusted
0.0 °C
Nearest neighbour
0.0 °C
Use the nearest-neighbour value for qPCR design; it accounts for salt, Mg²⁺, dNTPs and oligo concentration.
Sequence transforms
Reverse
—
Complement
—
Reverse complement
—