Calculators

Oligo dilution (C1V1 = C2V2)

Stock concentration (µM)Working concentration (µM)Final volume (µL)

Take 10.00 µL stock + 90.00 µL buffer

Amount of DNA (ng)Template length (bp)

1.853e+9 copies

Reaction volume (µL)Number of reactionsPipetting excess (%)

Prepare 220.0 µL total master mix