Resources

Tools and reference material for designing and running real-time PCR assays.

Primer & probe design

Paste a sequence and generate candidate primer/probe sets — entirely in your browser.

What every design parameter does, typical values, and how to read the results.

Tm, oligo dilution, copy number and master-mix volume calculators.

Cycling programs, handling and storage guidance for our assays.

Tm, molecular weight, extinction coefficient, OD conversions and structure checks.

IUPAC ambiguity codes, complements and a degenerate primer calculator.