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HBPP0001Single Tube Assay
Swine flu H1N1
In the pack
| No. | What's supplied | Volume per reaction | No. of vials |
|---|---|---|---|
| 1 | Swine flu H1N1 Primer Probe Mix | 5 µL | 1 |
| 2 | Positive Control Mix | 10 µL | 1 |
Specifications
- Cat. No.
- HBPP0001
- Target organisms
- Influenza A virus, Influenza B virus, Swine flu H1N1, RNase P (HEX)
- Storage
- -20C, protect from light
- Shipping
- Ambient (Gel Ice Packs)
- HSN code
- 38220090
- GST
- 5%
Assay channels
- Influenza A virusCY5-BHQ2
- Influenza B virusROX-BHQ2
- Swine flu H1N1FAM-BHQ1
- RNase P (HEX)HEX-BHQ1
Reaction setup
| Components | Volume |
|---|---|
| One Step RT PCR Master Mix | 8 µL |
| RT-Taq Mix | 2 µL |
| Swine flu H1N1 PP Mix | 5 µL |
| RNA | 10 µL |
| Total Reaction Volume | 25 µL |
Volumes are a starting point — scale to your instrument and plate format.
Recommended thermal profile
| No. of cycles | Step | Temperature | Time | Note |
|---|---|---|---|---|
| 1× | Reverse Transcription | 50C | 20min | |
| 1× | Taq Activation | 95C | 15min | |
| 40× | Denaturation | 95C | 20sec | |
| Annealing | 60C | 20sec | Data collection | |
| Extension | 72C | 20sec |
Profile is a starting point — optimise on your own instrument.
For research use only. Not for use in diagnostic procedures.